发明名称 |
PROCESSO DE PREPARACAO DE COMPOSICOES SACARIDEAS, DE COMPOSICOES FRAMACEUTICAS E PRODUTOS ALIMENTARES E APARELHO PARA A SINTESE DAS COMPOSICOES SACARIDEAS |
摘要 |
A method for preparing saccharide compositions is disclosed. The method is reiterative and comprises the following three steps.(i) A glycosyltransferase capable of transferring a preselected saccharide unit to an acceptor moiety is isolated by contacting the acceptor moiety with a mixture suspected of containing the glycosyltransferase under conditions effective to bind the acceptor moiety and the glycosyltransferase and thereby isolate the glycosyltransferase. The acceptor moiety is a protein, a glycoprotein, a lipid, a glycolipid, or a carbohydrate.(ii) The isolated glycosyltransferase is then used to catalyze the bond between the acceptor moiety and the preselected saccharide unit.(iii) Steps (i) and (ii) are repeated a plurality of times with the intermediate product obtained in the first iteration of the method being used as the acceptor moiety of the second iteration. |
申请公布号 |
PT97382(A) |
申请公布日期 |
1992.01.31 |
申请号 |
PT19910097382 |
申请日期 |
1991.04.16 |
申请人 |
THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA |
发明人 |
STEVEN ROTH |
分类号 |
C12N9/10;C12N11/00;C12P19/18;C12P19/26 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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