Monoclonal antibody recognizing the C-terminus of hBNP

摘要

A hybridoma producing a monoclonal antibody recognizing the C-terminus of human brain natriuretic peptide (hBNP) was cultivated in a medium or the abdominal cavity of a mouse to recover the monoclonal antibody from the medium or ascites accumulated in the abdominal cavity. An immunoassay of hBNP was established using the monoclonal antibody. The immunoassay of hBNP of this invention is so sensitive that the minimum detection limit is 1 pg/ml and can therefore determine an hBNP level in blood plasma directly, that is, without the extraction of hBNP from blood plasma. It is useful for diagnosing diseases such as hypertension and the like and states of heart, kidney and the like with taking an increase/decrease of an hBNP level as an index.