摘要 |
There are described novel porphyrin derivatives of the general formula <IMAGE> I wherein either R1 signifies the group <IMAGE> and R2 signifies the group <IMAGE> +TR <IMAGE> or R1 signifies the group <IMAGE> and R2 signifies the group <IMAGE> whereby X- signifies a halogen ion, an arylsulphonate ion, an alkylsulphonate ion or an alkyl sulphate ion, A signifies (C1-8)-alkylene and R3 signifies (C1-4)-alkyl, and sulphonic acid salts of compounds of formula I in which R1 signifies the group <IMAGE> as well as a process for their manufacture and intermediates used in this process. Because of their water-solubility the novel porphyrin derivatives are suitable as label molecules for highly sensitive fluorescence immuno-assays, especially for time-resolving fluorescence immuno-assays. The coupling of the novel porphyrin derivatives to immunologically-active materials is carried out in the customary manner, for example with a water-soluble carbodiimide derivative.
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