| 摘要 |
PROBLEM TO BE SOLVED: To provide products and processes for multiplex nucleic acid identification.SOLUTION: Products and processes for detecting the presence or absence of multiple target nucleic acids are provided in the specification. Certain methods include the steps of: amplifying the target nucleic acids, or portion thereof; extending oligonucleotides that specifically hybridize to the amplicons, where the extended oligonucleotides include a capture agent; capturing the extended oligonucleotides to a solid phase via the capture agent; releasing the extended oligonucleotide by competition with a competitor; and detecting the extended oligonucleotide, and thereby determining the presence or absence of each target nucleic acid by the presence or absence of the extended oligonucleotide.SELECTED DRAWING: None |
