Method of using a biosensor

摘要

A biosensor (102) for determining the presence or amount of a substance in a sample and methods of use of the biosensor (102) are provided. The biosensor (102) for receiving a user sample to be analyzed includes a mixture for electrochemical reaction with an analyte. The mixture includes an enzyme, a mediator and an oxidizable species as an internal reference.

主权项

1. A method of using a biosensor including a mixture of an only one enzyme, a mediator, and an oxidizable species as an internal reference, said oxidizable species being different than the mediator species and having different redox potentials, wherein said mixture is located directly on a surface of at least a portion of a working electrode, said method comprising the steps of:
applying a first voltage potential in a first voltage period; providing a set delay period; applying a second voltage potential in a second voltage period following said delay period; and wherein said first voltage potential and said second voltage potential are selectively provided for oxidizing only said mediator or both said mediator and said internal reference.