FLUID ANALYSIS DEVICE AND METHOD

摘要

The invention provides an analysis device (1) and method, that enables repeatedly pumping a sample fluid (50) with one or more analytes through a substrate (10) with one or more binding materials, even when the wetted substrate has a relatively low bubble pressure. Thereto, the device (1) comprises a first volume (16) and a second volume (18), and a return channel (22) with a valve (24) as well as a pump (20). The pump (20) can establish a pressure difference between the first volume (16) and the second volume (18), to pump the sample fluid (50) through the substrate (10), thereby allowing analyte to bind to the substrate. By opening the valve (24) and establishing a reversed pressure difference, sample fluid (50) bypasses the substrate (10) through the return channel (22). After closing the valve, the device is ready for a subsequent cycle, if desired. The device and method allow the analysis of in principle any amount of sample fluid, and the use of any type of substrate with respect to bubble pressure, and is thus more versatile and robust.