Apparatus for separating DNA molecules of chromosomal size by electrophoresis

摘要

This invention provides a method for fast resolving chromosome sized DNA molecules, and for obtaining reliable and reproducible DNA electrophoretic patterns. It is achieved by performing pulsed field minigel electrophoresis at a high electric field strength at expense of reducing the separation between opposed electrodes. This approach allows to increase the frequency needed to separate DNA molecules, diminishing the electrophoresis time. It also favors the reduction of the electrophoretic chamber, and the increase of the electric resistance in the chamber. This, together with the achievement of high electric field at low applied voltage, avoids the excess of heat generation in the electrophoretic chamber, allowing the use of simple cooling systems and power supplies. The method saves time, samples and reagents.