METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID

摘要

The purpose of the present invention is to increase the dissociation efficiency between biotin in a biotinylated nucleic acid and Tamavidin 2 in a Tamavidin 2-immobilized insoluble support to thereby efficiently obtain the biotinylated nucleic acid. Provided are: a method for obtaining a biotinylated nucleic acid, said method comprising "(1) a step (step A-1) for contacting a sample containing a biotinylated nucleic acid wherein biotin is bound to a nucleic acid with an insoluble support on which Tamavidin is immobilized (a Tamavidin-immobilized insoluble support) and thus forming a complex of the biotinylated nucleic acid and the Tamavidin-immobilized insoluble support, and (2) a step (step A-2) for separating the biotinylated nucleic acid from the complex obtained in step (A-1) in a solution of pH 7.8-9.5 in the presence of free biotin"; a method for separating a biotinylated nucleic acid to which a nucleic acid-binding protein is bound; a method for separating a nucleic acid-binding protein; and a kit for separating a nucleic acid.