RECOMBINANT CONSTRUCTS AND SYSTEMS FOR SECRETION OF PROTEINS VIA TYPE III SECRETION SYSTEMS

摘要

The present invention relates to a DNA construct that contains a first DNA molecule encoding a functional type III secretion system, a promoter, and a second DNA molecule encoding a protein or polypeptide capable of being secreted by the type III secretion system. The second DNA molecule is operably coupled to the promoter so that upon introduction of the DNA construct into a host cell, the encoded protein or polypeptide is secreted. Alternatively, the first and second DNA molecules can be on separate DNA constructs introduced into the same host cell. Methods of isolating a protein or polypeptide and identifying a gene encoding a potential effector protein or polypeptide are also disclosed.