Chemiluminescence protein chip, kit and method for detecting seroglycoid fucose index

摘要

H:/sxd/Intrwovn/NRPortbl/DCC/SXD/l0709684_ .doc-10/08/2016 Abstract The invention relates to a chemiluminescent protein chip, kit and method for detecting seroglycoid fucosylation index, belonging to protein detection technology. The chemiluminescent protein chip at least includes one detection subarea, and the detection subarea is used for detecting one serum sample; two detection spot areas and one row control spot area are arranged in the detection subarea, one of the detection spot areas contains detection spots formed from fixed alpha fetoprotein (AFP) specific antibodies, the other detection spot area contains detection spots formed from fixed lens culinaris lectin, and the control spot area contains control spots formed from fixed bovine serum albumin (BSA). In the method of the invention, on the chemiluminescent protein chip form "AFP McAb - AFP - biotin-labeled AFP PcAb - avidin HRP compound" and "lens culinaris lectin - AFP-L3 biotin-labeled AFP antibody - avidin HRP compound". The protein chip, kit and method of the invention are able to detect seroglycoid fucosylation index accurately with high throughput, and have the advantages of high sensitivity, time saving, convenient use and low cost in clinical application.