Sample distribution in a method for isolating analytes in fluid samples in an automated system

摘要

A method is described for distributing samples within an automated analyzer from a linear arrangement of sample vessels to a processing plate in a two-dimensional n×m arrangement wherein samples are sorted, followed by transfer with a pipetting device with a linear arrangement to a processing vessel in a two-dimensional n×m arrangement and subsequent processing of samples using a second pipetting device which has a two-dimensional n×m arrangement.

主权项

1. A method for isolating analytes that may be present in fluid samples in an automated system, the method comprising the automated steps of:
a) loading samples on the automated system with identifiers, or identifying loaded samples according to sample type and analytical test to be performed; b) transferring instructions from a control unit to a first and a second pipetting device comprising allocation of individual samples to a two-dimensional n×m arrangement of individual tests in a processing plate; c) transferring said fluid samples from at least two sample vessels arranged in a linear rack to a at least two vessels of said processing plate with a first pipetting device comprising at least two pipetting units in linear arrangement, wherein said pipetting units are coupled to pipette tips; d) combining together a solid support material and said fluid sample in a well of said processing plate for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material; e) isolating the solid support material from other material present in the fluid sample in a separation station; and f) purifying the analyte in the separation station by separating the fluid sample from the solid support material and washing the materials one or more times with a wash buffer, wherein said solid support material and said washing buffer are mixed by aspirating and dispensing with a second pipetting device, said second pipetting device comprising pipetting units in a two-dimensional n×m arrangement for coupling to pipette tips.