OPTIMIZED SMALL GUIDE RNAS AND METHODS OF USE

摘要

Methods, compositions, and kits are provided herein for CRISPER/Cas-mediated nucleic acid detection or modification.

主权项

1. A small guide RNA molecule comprising from 5′ to 3′:
a binding region, comprising between about 5 and about 50 nucleotides; a 5′ hairpin region, comprising:
fewer than four consecutive uracil nucleotides; ora length of at least 31 nucleotides; and a 3′ hairpin region; and a transcription termination sequence, wherein the small guide RNA is configured to form a complex with a small guide RNA-mediated nuclease, the complex having increased stability or activity relative to a complex containing a small guide RNA-mediated nuclease and a small guide RNA comprising at least 95% identity to SEQ ID NO:1 or a complement thereof.