SCREENING METHOD USING SOLID SUPPORTS MODIFIED WITH SELF-ASSEMBLED MONOLAYERS

摘要

Disclosed is a method for the identification of active compounds interacting with a target molecule, comprising the steps of: (a) forming a binding matrix comprising at least two different ligands on a solid support by immobilising said ligands via a common intermediate molecule on the support; (b) contacting a target of interest with said binding matrix; (c) parallely determining a binding value of the ligand/target interaction for each type of ligand comprised in the binding matrix; (d) selecting those ligands the binding value of which in an immobilised state with the target exceeds a predetermined threshold; (e) replacing the common intermediate molecule by a replacing fragment in order to form new ligands of increased molecular weight, with the replacing fragment being coupled to the ligand(s) of step (d) at the common binding position between the ligand and the intermediate molecule; (f) determining the affinity of the ligands formed in step (e) towards the target.