OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE

摘要

The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a beta-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a beta-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a beta-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a beta-lactmase or instructions for using the components in an assay.