DETECTION OF GENOME EDITING

摘要

Methods, compositions, and kits are provided for quantification of genome editing. In one aspect, the present invention provides a method for simultaneous quantification of homology directed repair (HDR) and non-homologous end joining (NHEJ) genome editing products in a sample of cells, wherein said sample of cells has been contacted with a site-specific genome editing reagent configured to cleave or nick DNA in a target enomic region and an HDR template nucleic acid.