METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS

摘要

The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

主权项

1. Oils, lipids and/or fatty acids produced by a transgenic Brassica plant, wherein said oils, lipids and/or fatty acids comprise 60 to 85% by weight of polyunsaturated fatty acids based on the total fatty acids in the transgenic plant, wherein said polyunsaturated fatty acids comprise at least 20% by weight of eicosapentaenoic acid (EPA), at least 2% by weight of docosapentaenoic acid (DPA), and/or at least 4% by weight of docosahexaenoic acid (DHA) based on the total fatty acids in the transgenic plant in form of triacylglycerides.