ISOLATED NUCLEOTIDE SEQUENCES ENCODING GDF TRAPS

摘要

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

主权项

1. A method for producing a variant ActRIIB polypeptide, comprising:
a) culturing a cell under conditions suitable for expression of the polypeptide, and b) recovering the polypeptide expressed by the cell; wherein the cell comprises a nucleic acid; wherein the nucleic acid comprises a nucleic acid sequence that hybridizes to the complement of nucleotides 76-396 of SEQ ID NO: 30 in the presence of 6.0×sodium chloride/sodium citrate (SSC) at 45° C. followed by a wash in 0.2×SSC at 65° C., wherein the nucleic acid sequence encodes a polypeptide that comprises an acidic amino acid at the position corresponding to position 79 of SEQ ID NO: 1.