发明名称 |
Assay method using NAD synthetase |
摘要 |
An assay method for a component in a specimen containing any one of ATP, deamide-NAD and an amide donor which comprises performing a main reaction which comprises incubating the specimen with NAD synthetase in the presence of ATP, deamide-NAD, an amide donor and Mg++ to generate NAD; performing a coenzyme cycling reaction by combining the oxidation-reduction reaction system with coenzyme NAD and the oxidation-reduction reaction system with coenzyme reduced NAD, and measuring a consumed or generated component in the cycling reaction. The NAD synthetase can be produced by culturing the microorganism Bacillus licheniformis B-0844 FERM P-6809, in a culture medium, and isolating the thus-produced NAD synthetase therefrom.
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申请公布号 |
US5206146(A) |
申请公布日期 |
1993.04.27 |
申请号 |
US19900481752 |
申请日期 |
1990.02.15 |
申请人 |
ASAHI KASEI KOGYO KABUSHIKI KAISHA |
发明人 |
MISAKI, HIDEO;ISHIKAWA, HIDEHIKO;MATSUURA, KAZUO |
分类号 |
C12N9/00;C12Q1/00;C12Q1/25;C12Q1/48;C12Q1/50 |
主分类号 |
C12N9/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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