METHODS FOR MAKING AND USING MODIFIED OOCYTES

摘要

The present invention provides modified oocytes having a nuclear genome derived from a first oocyte and cytoplasm derived from a second oocyte from a different subject, and methods for making and using such modified oocytes. The methods and compositions of the present invention can be useful in a variety of settings including, but not limited to, in in vitro fertilization (“IVF”) procedures.

主权项

1. A method for producing a modified oocyte, comprising:
a. obtaining a nuclear genome from a first oocyte, wherein the nuclear genome has not yet reached the mature metaphase II stage, or is an immature metaphase II nuclear genome, or is a metaphase II nuclear genome that has been treated to induce partial spindle depolymerization, b. removing the nuclear genome from a second oocyte to form an enucleated second oocyte, and c. introducing the nuclear genome from the first oocyte into the enucleated second oocyte to form a modified oocyte.