In vitro model for a tumor microenvironment

摘要

Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.

主权项

1. A method for mimicking a tumor microenvironment in vitro, the method comprising:
adding a culture medium to a cell culture container; plating at least one tumor cell type on a first surface of a porous membrane within the cell culture container; and indirectly applying a shear stress upon the at least one tumor cell type by applying the shear stress upon a second surface of the porous membrane, the shear stress resulting from flow of the culture medium induced by a flow device, the flow mimicking flow to which the tumor cells are indirectly exposed in vivo in the tumor microenvironment.