发明名称 |
COMPOSITIONS COMPRISING AAV EXPRESSING DUAL ANTIBODY CONSTRUCTS AND USES THEREOF |
摘要 |
A recombinant adeno-associated virus (AAV) having an AAV capsid and packaged therein a heterologous nucleic acid which expresses two functional antibody constructs in a cell is described. Also described are antibodies comprising a heavy chain and alight chain from a heterologous antibody. In one embodiment, the antibodies are co-expressed from a vector containing: a first expression cassette which encodes at least a first open reading frame (ORF) for a first immunoglobulin under the control of regulatory control sequences which direct expression thereof; and a second expression cassette which comprises a second ORF, a linker, and a third ORF under the control of regulatory control sequences which direct expression thereof, wherein the second and third ORF encode a second and third immunoglobulin construct. The vector co-expressing these two antibody constructs is in one embodiment an AAV, in which the 5′ and 3′ ITRs flank the expression cassettes and regulatory sequences. |
申请公布号 |
US2017081392(A1) |
申请公布日期 |
2017.03.23 |
申请号 |
US201515310555 |
申请日期 |
2015.05.13 |
申请人 |
The Trustees of the University of Pennsylvania |
发明人 |
Wilson James M.;Tretiakova Anna |
分类号 |
C07K16/10;A61K48/00;C12N7/00 |
主分类号 |
C07K16/10 |
代理机构 |
|
代理人 |
|
主权项 |
1. A recombinant adeno-associated virus (AAV) having an AM/capsid and packaged therein a heterologous nucleic acid which expresses at least two functional monospecific antibodies in a cell, wherein the recombinant AAV comprises:
a 5′ AAV inverted terminal repeat (ITR); a first expression cassette which encodes at least a first open reading frame (ORF) for a first immunoglobulin under the control of regulatory control sequences which direct expression thereof; a second expression cassette which comprises a second ORF, a linker, and a third ORF under the control of regulatory control sequences which direct expression thereof, wherein the second and third ORF are for a second and third immunoglobulin construct; and a 3′ AAV ITR. |
地址 |
Philadelphia PA US |