发明名称 |
IMPROVED BACTERIAL ENDOTOXIN TEST FOR THE DETERMINATION OF ENDOTOXINS |
摘要 |
Herein is reported a method for determining bacterial endotoxin at low concentrations in a sample of an antibody (that has been produced using bacterial cells) comprising the following steps in the following order: i) adding magnesium ions to the sample, ii) diluting the sample, iii) dialyzing the sample having a pH-value of 5.7-8.0 against an endotoxin-free aqueous solution, and iv) determining bacterial endotoxin in the sample using a bacterial endotoxin test, particularly the limulus amoebocyte lysate assay. |
申请公布号 |
EP3124976(A1) |
申请公布日期 |
2017.02.01 |
申请号 |
EP20150178683 |
申请日期 |
2015.07.28 |
申请人 |
F. Hoffmann-La Roche AG;Forschungszentrum Borstel Leibniz-Zentrum für Medizin und Biowissenschaften |
发明人 |
DEUTSCHMANN, Sven;LANG, Pierre;VON WINTZINGERODE, Friedrich;ALEXANDER, Christian;ZAEHRINGER, Ulrich |
分类号 |
G01N33/579 |
主分类号 |
G01N33/579 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|