Fusion proteins comprising an engineered knottin peptide and uses thereof
摘要
The present disclosure presents a general approach to engineering existing protein-protein interactions through domain addition and evolution. The disclosure teaches the creation of novel fusion proteins that include knottin peptides where a portion of the knottin peptide is replaced with a sequence that has been created for binding to a particular target. Such fusion proteins can also be bispecific or multi specific in that they can bind to and/or inhibit two or more receptors or receptor ligands. Knottins may be fused with an existing ligand (or receptor) as a general platform for increasing the affinity of a ligand-receptor interaction or for creating a multi specific protein. In addition, the fusion proteins may comprise a knottin peptide fused to another protein where the other protein facilitates proper expression and folding of the knottin.
申请公布号
AU2011326164(B2)
申请公布日期
2016.05.05
申请号
AU20110326164
申请日期
2011.11.07
申请人
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
发明人
COCHRAN, JENNIFER R.;JONES, DOUGLAS S.;KARIOLIS, MIHALIS S.;TSAI, PING-CHUAN