ENHANCED LUMINESCENCE FROM NANOPOLISHED SURFACE AND PLATE WELLS

摘要

The present invention relates to a method for fabricating a new silver coating/nanoparticle scaffold that significantly enhances the luminescence of near-field fluorophores via the metal enhanced fluorescence phenomenon. The silver coating/nanoparticle scaffold can be used for numerous applications in metal-enhanced fluorescence.

主权项

1. A substrate comprising a multiplicity of discrete metallic layers, wherein each discrete layer comprises discrete metallic nanoparticles thereby providing the substrate with enhanced luminescence, wherein the substrate is fabricated by the steps comprising:
a) providing a substrate for deposition of metallic layers; b) providing a metal containing solution, wherein the metal has plasmonic activity, contacting the metal containing solution with the substrate and heating same to a temperature of from about 30° C. to about 60° C. for a deposition time period ranging from 1 minute to 7 minutes to provide for deposition of metallic nanoparticles; c) transferring the heated substrate to a freezing chamber for a time period ranging from about 1 minute to 4 minutes to form a chilled substrate; d) transferring the chilled substrate back to a heating environment for a time period as in step b) or shorter than the previous heating period and in the same heating temperature range; e) removing the solution; and f) repeating steps b) and c) for at least one more time and optionally from 4 to 19 times to provide a multiplicity of layers of metallic nanoparticles, wherein the nanoparticles are discrete and substantially the same size.