Method for isolating cells

摘要

The present invention relates to a method for isolating cells from a complex sample comprising the steps of:
a) providing a complex sample,b) incubating said sample with:
at least one chaotrope,a buffer andat least one detergent,c) isolating said cells from the resulting mixture by centrifugation or filtration.

主权项

1. A method for isolating viable cells with cell walls from a complex sample comprising:
a) providing a complex sample, b) incubating said complex sample at 25° C. to 70° C. for at least 20 minutes with a lysis solution comprising:
at least one chaotrope in a concentration between 50 mM to 10M, that is urea, guanidine HCl, guanidine thiocyanate, guanidium thiosulfate, thiourea, sodium thiocyanate potassium thiocyanate, sodium iodide, sodium perchlorate or a combinations thereofa buffer having a pH of 6 to 10 and0.01% to 5% of at least one anionic, non-ionic and/or zwitterionic detergent until dissolution of said complex sample to form a mixture, c) subjecting the resultant mixture consisting of said complex sample and said lysis solution from incubation and dissolution step b) to a centrifugation or a filtration and isolating viable cells with cell walls from the resultant mixture whereby said viable cells with cell walls are retained on a filter after the filtration or retained in a pellet after the centrifugation.