| 摘要 |
PROBLEM TO BE SOLVED: To provide purification of Staphylococcus aureus type 5 and type 8 capsular saccharides.SOLUTION: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g., to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g., to remove low molecular weight contaminants; anion exchange chromatography, e.g., to remove residual protein; and concentration.SELECTED DRAWING: None |
