主权项 |
1. An analytic apparatus, comprising:
a casing, comprising at least one sample addition port in fluid communication with at least one detection region via at least one flow layer, at least one assay channel, and at least one control channel, wherein:
said at least one flow layer comprises at least one hydrophilic region and at least one hydrophobic barrier; and,said at least one hydrophilic region comprises at least one hydrophilic porous media configured to enable directional flow of fluids; at least one hydrophobic detection reagent provided within at least one hydrophilic region, wherein said at least one hydrophobic detection reagent is configured to convert from hydrophobic to hydrophilic upon contact with at least one chemical promoter and/or modify wetting properties of said at least one hydrophilic region upon contact with at least one chemical promoter; and, at least one responsive reagent provided within said at least one assay channel and/or said at least one control channel, wherein each at least one responsive reagent is configured, in response to presence and/or absence of at least one target analyte, to produce said at least one chemical promoter, not produce said at least one chemical promoter, or produce said at least one chemical promoter in amounts and/or at rates distinguishable within said at least one assay channel as compared to said at least one control channel; wherein said fluid moves at differential rates due to said differential amounts and/or rates of said chemical promoter produced within said at least one assay channel and/or said at least one control channel; and, wherein said apparatus is configured to determine a presence and/or concentration of said at least one target analyte within at least one sample when said at least one sample is introduced into said at least one a sample addition port. |