Vitro method for high throughput screening of genotoxic agents in eukaryotic cells

摘要

The present invention relates to a novel method for high throughput detection of wide range of genotoxins in eukaryotic cells wherein the eukaryotic cell based tool combines the ability to detect a broad spectrum of genotoxic signaling events and a simple and reproducible assay technique. The present invention further comprises expression cassettes, vectors, and eukaryotic cell lines for the same.

主权项

1. A eukaryotic cell comprising a first expression cassette which includes a DNA sequence encoding Renilla Luciferase (RLuc) and derivatives thereof as a reporter protein, operatively linked to human p21 gene promoter; a second expression cassette which includes a DNA sequence encoding humanized Firefly Luciferase (hFLuc) and derivatives thereof as a reporter protein, operatively linked to hamster GADD153 gene promoter; and a third expression cassette which includes a DNA sequence encoding beta galactosidase (Bgal) and derivatives thereof as reporter protein, operatively linked to p53 response element and polyoma virus UTR as promoter.