Flow cytometry for high throughput screening

摘要

The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.

主权项

1. A method for analyzing a plurality of samples, comprising:
moving a plurality of samples comprising particles in a flow stream to and through a flow cytometer, said flow stream including a separation fluid between adjacent ones of said plurality of samples separating said samples from each other in said flow stream, said flow stream thereby constituting a fluid-separated sample flow stream; and operating said flow cytometer to focus and detect said plurality of samples in said fluid-separated sample flow stream and to selectively analyze the particles in each of said plurality of samples as said fluid-separated sample flow stream passes through said flow cytometer, the operating of said flow cytometer including focusing and detecting said separation fluid as different components from said samples.