发明名称 |
METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS |
摘要 |
Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis. |
申请公布号 |
US2016025761(A1) |
申请公布日期 |
2016.01.28 |
申请号 |
US201414775685 |
申请日期 |
2014.03.14 |
申请人 |
FLUIDIGM CORPORATION |
发明人 |
West Jason A. A.;Fowler Brian |
分类号 |
G01N35/08;B01L3/00;C12M1/04;C12M1/00;G01N33/50;G01N33/569 |
主分类号 |
G01N35/08 |
代理机构 |
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代理人 |
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主权项 |
1. A method for cell analysis, comprising carrying out at least two rounds of cell capturing, characterization, and transport, each round comprising:
a) flowing a solution comprising a plurality of individual cells (and/or cellular units) into a first microfluidic channel; b) partitioning the channel into a plurality of contiguous segments (S), thereby capturing at least one cell in at least one segment, wherein
i) one or more of said segments comprises a single captured cell (and/or single captured cellular unit), and c) determining at least one characteristic of one or more of said single captured cells and/or cellular units; and d) independently transporting each said captured cell or unit to a specified cell holding chamber based on the determined characteristic, whereby for each specified destination chamber the characteristic(s) of cell(s) transported thereto is known. |
地址 |
South San Francisco CA US |