| 摘要 |
The present invention relates to a method for fine-tuning gene expression in a prokaryote using a synthetic regulatory small RNA (sRNA), and more specifically, to a synthetic regulatory sRNA which can fine-tune target gene expression by adjusting an expression amount of the sRNA or a binding force between the sRNA and Hfq to fine-tune target gene expression. According to the present invention, the method for fine-tuning gene expression using the synthetic regulatory sRNA adjusts the expression amount of the sRNA or the binding force between the sRNA and Hfq to fine tune a suppression degree of the target gene expression to apply a combination of various target genes to various strains simultaneously, easily, and quickly without a gene deletion process through the synthetic regulatory sRNA which regulates gene expression, which is very suitable for measuring a metabolic capability for each strain and selecting an optimal strain. Also, a target gene for gene expression suppression can be selected easily and quickly, and the selected target gene can be expressed to a desired degree. Therefore, the method can be used in constructing a recombinant strain for efficiently producing various metabolites and establishing an efficient production method. |
