发明名称 |
SIMULTANEOUS QUANTIFICATION OF NUCLEIC ACIDS IN DISEASED CELLS |
摘要 |
Processes and methods for the simultaneous quantification of nucleic acids in diseased cells that are based on real-time PCR are provided. The real-time PCR protocol is an excellent tool for reliable quantification of in vitro drug screening and evaluation protocols to determine the efficacy of potential anti-viral agents. Quantification using these simultaneous PCR cycle threshold (Ct) detection techniques during one-step real-time RT-PCR (Applied Biosystems, CA) eliminates the variability resulting from quantification of end-point RT-PCR products. In addition, the mitochondrial toxicity assay is an added tool to assess potential side-effects for these chemotherapeutic agents.
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申请公布号 |
US2009220950(A1) |
申请公布日期 |
2009.09.03 |
申请号 |
US20070949162 |
申请日期 |
2007.12.03 |
申请人 |
PHARMASSET, INC. |
发明人 |
STUYVER LIEVEN;OTTO MICHAEL J. |
分类号 |
C12N15/09;C12Q1/68;C07H19/048;C07H19/06;C07H19/10;C07H19/16;C07H19/20;C07H21/04;C12Q1/70 |
主分类号 |
C12N15/09 |
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