COMBINED METHODOLOGY USED TO DETECT THE PRESENCE OF POSSIBLE CONTAMINATING EVENTS

摘要

Disclosed herein are methods for determining if a contaminating integration of a nucleotide sequence is present in a set of nucleic acids. Further disclosed herein are methods for determining the copy number/zygosity of a nucleic acid sequence of interest. The methods disclosed herein may be performed using quantitative PCR.

主权项

1. A method of determining if a contaminating integration of a nucleotide sequence is present in a set of nucleic acid, the method comprising:
performing a first assay to provide a first value indicative of the total number of iterations of the nucleotide sequence in the set of nucleic acid; performing a second assay to provide a second value indicative of the number of iterations of the nucleotide sequence at a particular integration site in the set of nucleic acid; and comparing the first value to the second value; wherein the first value being greater than the second value indicates the presence of a contaminating integration.