| 主权项 |
1. A method for amplifying a nucleic acid sample, comprising:
a) providing a support comprising a first primer and a second primer; b) coupling the first primer to a first single-stranded nucleic acid molecule that is derived from the nucleic acid sample; c) performing a first primer extension reaction using the first primer to generate a first double-stranded nucleic acid molecule comprising the first single-stranded nucleic acid molecule and a second single-stranded nucleic acid molecule that is complementary to at least a portion of the first single-stranded nucleic acid molecule; d) at least partially denaturing the first double-stranded nucleic acid molecule by binding an invader species to at least a portion of the first or second single-stranded nucleic acid molecule, wherein the invader species is different from the second primer, which binding exposes a segment of the first single-stranded nucleic acid molecule that is complementary to the second primer; and e) coupling the second primer to the segment of the first single-stranded nucleic acid molecule and performing a second primer extension reaction using the second primer to generate a second double-stranded nucleic acid molecule comprising the first single-stranded nucleic acid molecule and a third single-stranded nucleic acid molecule that is complementary to at least a portion of the first single-stranded nucleic acid molecule, wherein the second primer extension reaction separates the second single-stranded nucleic acid molecule from the first single-stranded nucleic acid molecule. |
