发明名称 |
METHODS AND COMBINATIONS OF SIGNALING MARKERS FOR ASSESSMENT OF DISEASE STATES |
摘要 |
Methods for preparation of cells for analysis of biomarkers are disclosed. In one aspect, the method for preparation of cells for analysis of biomarkers includes contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; and contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population. |
申请公布号 |
US2015301032(A1) |
申请公布日期 |
2015.10.22 |
申请号 |
US201314647414 |
申请日期 |
2013.11.27 |
申请人 |
DEEPATH MEDICAL INC. |
发明人 |
DE Jita |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
1. A method for preparation of cells for analysis of biomarkers, comprising:
(a) contacting a sample that contains a population of cells with at least one modulating substance at a first temperature, thereby producing a modulated cell population; (b) contacting the modulated cell population with at least one antibody that is directed to a cell surface biomarker at a second temperature that is lower than the first temperature, thereby producing an extracellularly stained cell population; (c) contacting the extracellularly stained cell population with one or more reagents that fixes and permeabilizes the cells, thereby producing a fixed and permeabilized cell population. |
地址 |
Palo Alto CA US |