MAGNETIC NANOPARTICLE AND ADENO-ASSOCIATED VIRUS COMPOSITE, AND GENE DELIVERY TO CELL USING THE SAME

摘要

PURPOSE: A composite of magnetic nanoparticles and adeno-associated virus, and a gene delivery method into a cell using the same are provided to deliver a foreign gene into a non-invasive cell type, to control moving speed of a magnet, and to control foreign gene delivery speed. CONSTITUTION: A composite contains superparamagnetic nanoparticles which are conjugated through a streptavidin-biotin-NTA(nitrilotriacetic acid)-nickel-hexa histidine linker, and an adeno-associated virus(AAV) vector. The superparamagnetic nanoparticles are gamma-Fe2O3, Fe3O4, or a mixture thereof. The adeno-associated virus vector is AAV2 vector containing cytomegalovirus(CMV) promoter and hexa histidine at a 587th site of LQRGNLGHHHHHHSRQA-(corresponding to wild type AAV2:^583 LQRGNRQA). A gene delivery method into a cell comprises: a step of delivering a gene delivery system into the non-invasive cell type using the magnet; and a step of controlling moving speed of the magnet for controlling gene delivery speed. [Reference numerals] (AA) Streptavidin; (BB) Biotin; (CC) Hexa histidine