| 发明名称 | Purification of vaccinia viruses using hydrophobic interaction chromatography | ||
| 摘要 | The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production. | ||
| 申请公布号 | US9109201(B2) | 申请公布日期 | 2015.08.18 |
| 申请号 | US201313906541 | 申请日期 | 2013.05.31 |
| 申请人 | Bavarian Nordic A/S;Otto-von-Guericke-Universität;Sartorius Stedim Biotech GmbH | 发明人 | Post Hansen Sara;Faber Rene;Reichl Udo;Wolff Michael;Gram Anders Peter |
| 分类号 | C12N7/01;C12N7/00;C12N15/863;C12N7/02;C12N15/86;A61K39/00 | 主分类号 | C12N7/01 |
| 代理机构 | Law Office of Salvatore Arrigo and Scott Lee, LLP | 代理人 | Law Office of Salvatore Arrigo and Scott Lee, LLP |
| 主权项 | 1. A purified recombinant vaccinia virus preparation comprising 10-100 ng of cellular DNA per 108 TCID50 of the recombinant vaccinia virus, wherein the vaccinia virus preparation has not been treated with a nuclease and the recombinant vaccinia virus comprises a piece of foreign genetic material that has been inserted into the viral genome. | ||
| 地址 | Kvistgaard DK | ||