| 摘要 |
The present invention refers to a process for generating a functional dermal substitute with pigmentation and hair follicles and use thereof as a regenerative agent, which is obtained from the culture in vitro of human skin cells to be combined with gelling biodegradable and bio-compatible matrixes applied to diabetic foot, burns and skin cancer. The process comprises the following stages: obtaining a silicon resin solution, incubating the silicon resin solution, separating the dermis and epidermis, obtaining melanocytes, incubating the epidermis layer, disintegrating the dermis layer, disintegrating the epidermis layer, proliferating the keratinocytes in suspension, obtaining follicle dermal cells, separating the sterile biomass, generating cellular aggregates, preparing the functional dermal substitute and inoculating and proliferating the keratinocytes and melanocytes in the primary dermal substitute. |
