NUCLEIC ACID ANALYSIS DEVICE

摘要

A nucleic acid analysis device which can determine a DNA sequence has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized. At least a part of the flowcell is made of a transparent material. An irradiation unit irradiates a part in which the DNA fragment clusters are immobilized. The device has a lens for collecting fluorescence, and a light-detection element. A solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution are sent sequentially to where the DNA fragment clusters are immobilized.

主权项

1. A nucleic acid analysis device having:
a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized, wherein at least a part of the flowcell is made of a transparent material; an irradiation unit for irradiating a part in which the DNA fragment clusters are immobilized; a lens for collecting fluorescence; and a light-detection element for detecting a collected light: which is characterized in that a solution containing dATP having a fluorescently modified phosphate terminal among four bases, a solution containing dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution for washing out the bases are sent sequentially to the part in which the DNA fragment clusters are immobilized.