NOVEL METHOD FOR SEARCHING AND QUANTIFYING CELLULASE USING REDESIGNED GENETIC CIRCUIT

摘要

The present invention relates to a novel method for searching cellulase using a redesigned genetic circuit and, more specifically, to a novel method for searching cellulase using a redesigned genetic circuit and a method for quantifying cellobiose degraded by the cellulase. When the redesigned genetic circuit of the present invention is used, the cellobiose recognition transcription regulation protein (CelR), which is proportional to the amount of cellobiose, induces the expression of a reporter protein, thereby promptly sensing and quantifying cellobiose with high sensitivity, and thus the present invention can be utilized for cellobiose sensing and quantitative analysis, and enables the measurement of the activity of exo-type cellulase which frees cellobiose and the search of new exo-type cellulase.