Compositions and methods for detection of <i>Staphylococcus aureus</i>

摘要

The present invention relates to methods for the rapid detection of the presence or absence of Staphylococcus aureus in a biological or nonbiological sample. The present invention includes methods of detection comprising performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, the present invention relates to primers, probes, and kits that are designed for the detection of Staphylococcus aureus.

主权项

1. A method of detecting Staphylococcus aureus (SA) in a sample, the method comprising:
performing an amplifying step comprising contacting the sample with a set of SA primers to produce an amplification product if SA is present in the sample whether or not SA is methicillin resistant; performing a hybridizing step comprising contacting the amplification product with one or more detectable SA probes; and detecting the presence or absence of the amplified product, wherein the presence of the amplified product is indicative of the presence of SA in the sample whether or not SA is methicillin resistant, and wherein the absence of the amplified product is indicative of the absence of SA in the sample whether or not SA is methicillin resistant; wherein the set of SA primers comprise a first primer consisting of the sequence of SEQ ID NO: 9, or the complete complement thereof, and a second primer consisting of the sequence of SEQ ID NO: 12, or the complete complement thereof, wherein the amplification product produced by the set of SA primers consist of the sequence of SEQ ID NO: 13, or the complete complement thereof; and wherein the one or more detectable SA probes comprise at least one detectable SA probe consisting of the sequence of SEQ ID NO: 10, or the complete complement thereof.