METHOD FOR CHROMATOGRAPHY REUSE

摘要

The present invention provides methods for cleaning or regenerating a chromatography materiel for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products.

主权项

1. A method to clean a chromatography material for reuse, the method comprising the steps of
a) passing two or more material volumes of elution buffer through the material, wherein the elution buffer comprises about 0.15 M acetic acid and is about pH 2.9; b) statically holding the material in elution buffer for a time ranging from about 10 minutes to about 30 minutes; c) passing about two or more material volumes of elution buffer through the material; and d) passing about two or more material volumes of regeneration buffer through the material, wherein the regeneration buffer comprises about 0.1 N NaOH and is about pH 13.