Method for screening phage display libraries against each other

摘要

The present invention relates to a method for screening phage display libraries against each other. In particular, the invention relates to a method for screening at least two phage display libraries against each other to identify and/or select one or more interacting binding partners or binding molecules making up such interacting binding partners. Kits providing two bispecific phage display libraries are also provided.

主权项

1. A method for screening at least two phage display libraries against each other to identify and/or select one or more interacting binding partners comprising:
(a) providing a first phage display library, wherein phages of said first phage display library are bispecific and comprise a member of a first library displayed at a first position and a first tag displayed at a second position; (b) contacting said first bispecific phage display library with a second bispecific phage display library under conditions permitting binding between members of the first bispecific phage display library and the second bispecific phage display library to form library 1-library 2 complexes, wherein phages of said second phage display library comprise a member of a second library displayed at a first position and a second tag displayed at a second position, wherein
(i) said first library and second library displayed at the pIII position on the phage particles and the first and second tag molecules are displayed at the pVII position on the phage particles; or(ii) said first library is displayed at the pIII position and the first tag is displayed at the pVII position on the phage particles and wherein said second library is displayed at the pVII position and the second tag is displayed at the pIII position on the phage particles; or(iii) said first library is displayed at the pVII position and the first tag is displayed at the pIII position on the phage particles and wherein said second library is displayed at the pIII position and the second tag is displayed at the pVII position on the phage particles; or(iv) said first library and second library are displayed at the pVII position on the phage particles and the tag molecules are displayed at the pIII position on the phage particles; and wherein the first and second s are different; and (c) separating library 1-library 2 complexes from unbound first bispecific phage display library members or unbound second bispecific phage display library members wherein the separation step (c) is carried out by one or more steps which comprise immobilizing either the first phage display library or the second phage display library onto a solid phase by way of said tags displayed on the phage display libraries, and wherein the one or more steps which comprise immobilizing are carried out before or after step (b).