| 摘要 |
FIELD: medicine.SUBSTANCE: invention aims at a method for identifying microorganisms from a test blood culture sample. The method provides preparing a test sample, conducting a selective lysis and dissolving the cells other than microorganisms of the test sample, laminating the prepared lysate on a density buffer in a hermetically sealed container and centrifuging. The density buffer has a uniform density from approximately 1.025 g/ml to approximately 1.120 g/ml. The microorganisms passing through the above buffer are precipitated on a container bottom. The precipitate is analysed with the use of Raman spectroscopy that enables identifying a genus or a species of the microorganism.EFFECT: invention enables identifying the microorganisms from the clinical samples over less than 120 min.13 cl, 4 dwg, 3 ex |
