Humanized anti-IL 10 antibodies for the treatment of systemic lupus erythematosus (SLE)

摘要

Provided is a humanized or chimeric antibody or fragment thereof capable of binding to interleukin-10 (IL-10), wherein said antibody or fragment thereof: (i) binds to the same region of IL-10 as the IL-10 receptor α(IL-I10Ra) and is not capable of binding IL-10 when the IL-10 is bound to the IL- 10 receptor; and (ii) binds to IL-10 in homodimeric form by binding a discontinuous epitope comprising residues of both monomers. Further provided are related products and methods involving the use of the antibody or fragment thereof.

主权项

1. A humanized or antibody or fragment thereof capable of binding to interleukin-10 (IL-10), wherein the antibody or fragment thereof comprises a variable light chain comprising the amino acid sequences of CDR1, CDR2 and CDR3 of the murine antibody B-N10 variable light chain, and a variable heavy chain comprising the amino acid sequences of CDR1, CDR2 and CDR3 of the murine antibody B-N 10 variable heavy chain, wherein the variable heavy chain further comprises the residues Asn73 and Ser74 of SEQ ID NO:70 or comprise the residues Asn and Ser, respectively, in the heavy chain at the seventh and eighth positions C-terminal to the heavy chain CDR2 which contact IL-10 when said antibody binds to IL-10.