主权项 |
1. A method for synthesizing slow and controlled release of a buprenorphine entrapped in nanoparticle or nanocarrier, the method comprising steps of:
dissolving a buprenorphine in an organic solvent to get an organic solution, wherein the buprenorphine is dissolved at a concentration of 0.1 to 10 mg/ml in the organic solvent, and wherein a concentration of the organic solvent is 0.1 to 5 mg/ml; adding a phospholipid to the organic solution to form a bilayer around the buprenorphine; adding a lipid to the organic solution to obtain an organic solution mixture, wherein the organic solution mixture comprises organic solvent with the buprenorphine, phospholipid and lipid; adding the organic solution mixture by dropwise to an aqueous solution to form a lipid core, and wherein the aqueous solution is a water or a buffer, and wherein pH of the aqueous solution is in a range of 3-11, and wherein a volume ratio of the organic solution mixture to the aqueous solution is within 0.05 to 3; and adding a buffered solution of a polymer to the aqueous solution with lipid core to form a coating layer around the lipid core to obtain a nano carrier, and wherein pH of the polymer solution is in a range of 3.5 to 11, and wherein the polymer solution is added dropwise to the aqueous solution in a volume ratio of 0.05 to 1. |