| 发明名称 | Methods for detecting vitamin D metabolites by mass spectrometry | ||
| 摘要 | Provided are methods of detecting the presence or amount of a vitamin D metabolite in a sample using mass spectrometry. The methods generally comprise ionizing a vitamin D metabolite in a sample and detecting the amount of the ion to determine the presence or amount of the vitamin D metabolite in the sample. Also provided are methods to detect the presence or amount of two or more vitamin D metabolites in a single assay. | ||
| 申请公布号 | US8936943(B2) | 申请公布日期 | 2015.01.20 |
| 申请号 | US201313871457 | 申请日期 | 2013.04.26 |
| 申请人 | Quest Diagnostics Investments, Inc. | 发明人 | Clarke Nigel J.;Holmquist Brett;Lee Gloria Kwang-Ja;Reitz Richard E. |
| 分类号 | G01N24/00;G01N33/82;G01N30/72;G01N30/88;H01J49/00 | 主分类号 | G01N24/00 |
| 代理机构 | Quest Diagnostics, Inc. | 代理人 | Quest Diagnostics, Inc. |
| 主权项 | 1. A method for determining the amount of a vitamin D metabolite in a sample by tandem mass spectrometry, comprising: (a) generating a protonated and dehydrated precursor ion of the vitamin D metabolite; (b) generating one or more fragment ions of the precursor ion; (c) detecting the amount of one or more of the ions generated in step (a) or (b) or both and relating the detected ions to the amount of the vitamin D metabolite in the sample; and (d) comparing the detected amount of the one or more ions with an internal standard to determine the amount of the vitamin D metabolite in the sample. | ||
| 地址 | Wilmington DE US | ||