Reagents and methods for direct labeling of nucleotides

摘要

The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration.

主权项

1. A method for direct labeling of an analyte comprising:
a. contacting a protected labeling compound with a polymer-based activating reagent having a polymer-bound moiety in a reaction mixture to generate a reactive labeling compound having a different density from said protected labeling compound and said activating reagent; b. separating said reactive labeling compound from said reaction mixture, c. contacting said reactive labeling compound with an analyte having a nucleophilic group capable of reacting with said reactive labeling compound; wherein the protected labeling compound comprises the structure: