Immunogen platform

摘要

Aspects of the present invention relate to chimeric polypeptides including HCV NS3/4A sequences and T-cell epitopes. Embodiments include nucleic acids encoding the chimeric NS3/4A polypeptides, the encoded polypeptides, compositions containing said nucleic acids, compositions containing said chimeric polypeptides, as well as methods of making and using the aforementioned compositions including, but not limited to medicaments and vaccines.

主权项

1. An immunogenic composition comprising a nucleic acid molecule that comprises a nucleotide sequence encoding a heterologous chimeric hepatitis viral antigen comprising hepatitis C virus (HCV) NS3/4A antigen fused to a hepatitis B virus (HBV) core antigen, wherein the nucleotide sequence is modified to encode an amino acid sequence that comprises one or more protease cleavage site insertions at one or more non-naturally occurring positions and said immunogenic composition induces an effective cytotoxic T cell (CTL) immune response specific for one or more epitopes of said heterologous viral antigen.