| 摘要 |
[Problem] Based on the prediction that the rules for DNA recognition possessed by a PPR motif may also be used in DNA recognition, perform analysis of PPR protein that is active in DNA binding, and search for a PPR protein that has such characteristics. The present invention addresses the aforementioned problem by means of a protein that can bind DNA base-selectively or DNA base sequence-specifically, and that contains a plurality of, preferably 2-30, more preferably 5-25, and even more preferably 9-15, PPR motifs having the structure in formula 1 (in formula 1: Helix A is a part that can form an α helix structure; X is a part that does not exist or that comprises 1-9 amino acids; Helix B is a part that can form an α helix structure; and L is a part that comprises 2-7 amino acids), the PPR motif having a specific combination of three amino acids corresponding to the DNA base or target base sequence: first A.A. of Helix A and the 4th A.A. of Helix A in formula 1, together with the "ii"(-2)th A.A. contained in L. (Helix A)-X-(Helix B)-L (formula 1) |
